rabbit anti mouse primary antibodies against hif 1α  (Proteintech)

Journal: International Journal of Environmental Research and Public Health

Article Title: Inhibition of HIF-1α Attenuates Silica-Induced Pulmonary Fibrosis

doi: 10.3390/ijerph19116775

Figure Lengend Snippet: Sequences of the primers.

Article Snippet: For IHC staining, refer to the papers [ ]; 5 μm thick sections were incubated with rabbit anti-mouse primary antibodies against HIF-1α (1:200, 20960-1-AP, ProteinTech) and COL1A1 (1:1000, GB11022-3, Servicebio), followed by horseradish peroxidase (HRP)–conjugated goat anti-rabbit IgG secondary antibody (1:8000, SA00001-2, Proteintech).

Techniques:

Journal: International Journal of Environmental Research and Public Health

Article Title: Inhibition of HIF-1α Attenuates Silica-Induced Pulmonary Fibrosis

doi: 10.3390/ijerph19116775

Figure Lengend Snippet: The effect of TGF-β1 on cell transdifferentiation was analyzed by comparing the expression of COL1A1, HIF-1α, and α-SMA using TGF-β1-treated cells at different concentrations. ( A ) Western blot analysis of col1a1, HIF-1α, and α-SMA in cells of different groups. β-actin was used as loading control. ( B ) Quantitative analysis of COL1A1, HIF-1α, and α-SMA is shown in A. ( C ) Cells were treated with TGF-β1 at different concentrations, and mRNA was extracted from the treated groups. mRNA expression was determined by qPCR and normalized to β-actin. * p < 0.05 vs. 0 ng/mL group; # p < 0.05 vs. 2.5 ng/mL group; & p < 0.05 vs. 5 ng/mL group; % p < 0.05 vs. 7.5 ng/mL group. Results are expressed as means ± SD. ANOVA was used to compare multiple groups and pairwise comparisons were compared by LSD-t test. A value of p < 0.05 was considered to be statistically significant.

Article Snippet: For IHC staining, refer to the papers [ ]; 5 μm thick sections were incubated with rabbit anti-mouse primary antibodies against HIF-1α (1:200, 20960-1-AP, ProteinTech) and COL1A1 (1:1000, GB11022-3, Servicebio), followed by horseradish peroxidase (HRP)–conjugated goat anti-rabbit IgG secondary antibody (1:8000, SA00001-2, Proteintech).

Techniques: Expressing, Western Blot, Control

Journal: International Journal of Environmental Research and Public Health

Article Title: Inhibition of HIF-1α Attenuates Silica-Induced Pulmonary Fibrosis

doi: 10.3390/ijerph19116775

Figure Lengend Snippet: The effect of TGF-β1 on cell transdifferentiation was analyzed by comparing the expression of COL1A1, HIF-1α, and α-SMA using TGF-β1-treated cells for different times. ( A ) Western blot analysis of COL1A1, HIF-1α, and α-SMA in cells of different groups. β-actin was used as a loading control. ( B ) Quantitative analysis of COL1A1, HIF-1α, and α-SMA is shown in A. ( C ) Cells were treated with TGF-β1 at different times, and mRNA was extracted from the treated groups. mRNA expression was determined by qPCR and normalized to β-actin. * p < 0.05 vs. 0 h group; # p < 0.05 vs. 2 h group; % p < 0.05 vs. 4 h group; $ p < 0.05 vs. 6 h group; ** < 0.05 vs. 12 h group. ANOVA was used to compare multiple groups and pairwise comparisons were compared by LSD-t test. A value of p < 0.05 was considered to be statistically significant.

Article Snippet: For IHC staining, refer to the papers [ ]; 5 μm thick sections were incubated with rabbit anti-mouse primary antibodies against HIF-1α (1:200, 20960-1-AP, ProteinTech) and COL1A1 (1:1000, GB11022-3, Servicebio), followed by horseradish peroxidase (HRP)–conjugated goat anti-rabbit IgG secondary antibody (1:8000, SA00001-2, Proteintech).

Techniques: Expressing, Western Blot, Control

Journal: International Journal of Environmental Research and Public Health

Article Title: Inhibition of HIF-1α Attenuates Silica-Induced Pulmonary Fibrosis

doi: 10.3390/ijerph19116775

Figure Lengend Snippet: The effect of TGF-β1 on cell transdifferentiation was analyzed by comparing the expression of HIF-1α, SMAD 3, and PSMAD3 using TGF-β1-treated cells. ( A ) Western blot analysis of HIF-1α, SMAD 3, and PSMAD3 in cells of different groups. β-actin was used as a loading control. ( B ) Quantitative analysis of HIF-1α, P SMAD 3/ SMAD 3 is shown in A. * p < 0.05 vs. 0 h group; # p < 0.05 vs. 2 h group; % p < 0.05 vs. 4 h group. ANOVA was used to compare multiple groups and pairwise comparisons were compared by LSD-t test. A value of p < 0.05 was considered to be statistically significant.

Article Snippet: For IHC staining, refer to the papers [ ]; 5 μm thick sections were incubated with rabbit anti-mouse primary antibodies against HIF-1α (1:200, 20960-1-AP, ProteinTech) and COL1A1 (1:1000, GB11022-3, Servicebio), followed by horseradish peroxidase (HRP)–conjugated goat anti-rabbit IgG secondary antibody (1:8000, SA00001-2, Proteintech).

Techniques: Expressing, Western Blot, Control

Journal: International Journal of Environmental Research and Public Health

Article Title: Inhibition of HIF-1α Attenuates Silica-Induced Pulmonary Fibrosis

doi: 10.3390/ijerph19116775

Figure Lengend Snippet: Cells were treated with DMSO, DMOG, and KC7F2 in the presence or absence of TGF-β1. mRNA and protein expression were examined to infer the degree of transdifferentiation. TGF-β1 was not present in the control group, the DMOG group, and the KC7F2 group. TGF-β1 was present in all of the TGF-β1 groups, the TGF-β1 + DMOG group, and the TGF-β1 + KC7F2 group. ( A ) Western blot analysis of COL1A1, HIF-1α, α-SMA, SMAD 3, and PSMAD3 in cells of different groups. β-actin was used as loading control. ( B ) Quantitative analysis of COL1A1, HIF-1α, P SMAD 3/ SMAD 3, and α-SMA is shown in A. ( C ) Different reagents were used to treat different groups of cells, and mRNA was extracted from groups. mRNA expression was determined by qPCR and normalized to β-actin. * p < 0.05 vs. control group; # p < 0.05 vs. DMOG group; $ p < 0.05 vs. TGF-β1 group. Results are expressed as means ± SD. ANOVA was used to compare multiple groups and pairwise comparisons were compared by LSD-t test. A value of p < 0.05 was considered to be statistically significant.

Article Snippet: For IHC staining, refer to the papers [ ]; 5 μm thick sections were incubated with rabbit anti-mouse primary antibodies against HIF-1α (1:200, 20960-1-AP, ProteinTech) and COL1A1 (1:1000, GB11022-3, Servicebio), followed by horseradish peroxidase (HRP)–conjugated goat anti-rabbit IgG secondary antibody (1:8000, SA00001-2, Proteintech).

Techniques: Expressing, Control, Western Blot

Journal: International Journal of Environmental Research and Public Health

Article Title: Inhibition of HIF-1α Attenuates Silica-Induced Pulmonary Fibrosis

doi: 10.3390/ijerph19116775

Figure Lengend Snippet: Cells were treated with NC-siRNA and HIF-1α-siRNA in the presence or absence of TGF-β1 and changes in mRNA and protein were examined to infer the degree of transdifferentiation. ( A ) Using flow cytometry, cell fluorescence intensity (transfection of siRNA that does not carry fluorescent groups) was measured, and 99.3% of the fluorescence intensity range was set as the threshold value (below which the cells were considered not to carry fluorescent groups), and transfection efficiency was calculated based on this threshold value. According to the threshold, cells exceeding the threshold were identified as successfully transfected with HIF-1α-siRNA (carrying fluorescent moieties). (HIF-1α-siRNA transfection efficiency was 71.9%) ( B ) Western blot analysis of COL1A1, HIF-1α,α-SMA, SMAD 3, and PSMAD3 in cells of different groups. β-actin was used as loading control. ( C ) Quantitative analysis of α-SMA, COL1A1, P SMAD 3/ SMAD 3, and HIF-1αis shown in B. ( D ) mRNA was extracted from the different groups, determined by qPCR, and normalized to β-actin. * p < 0.05 vs. NC-siRNA group; $ p < 0.05 vs. TGF-β1 + NC-siRNA group. Results are expressed as means ± SD. ANOVA was used to compare multiple groups and pairwise comparisons were compared by LSD-t test. A value of p < 0.05 was considered to be statistically significant.

Article Snippet: For IHC staining, refer to the papers [ ]; 5 μm thick sections were incubated with rabbit anti-mouse primary antibodies against HIF-1α (1:200, 20960-1-AP, ProteinTech) and COL1A1 (1:1000, GB11022-3, Servicebio), followed by horseradish peroxidase (HRP)–conjugated goat anti-rabbit IgG secondary antibody (1:8000, SA00001-2, Proteintech).

Techniques: Flow Cytometry, Fluorescence, Transfection, Western Blot, Control

Journal: International Journal of Environmental Research and Public Health

Article Title: Inhibition of HIF-1α Attenuates Silica-Induced Pulmonary Fibrosis

doi: 10.3390/ijerph19116775

Figure Lengend Snippet: Cells were treated with DMSO, DMOG, SIS3, and SIS3 + DMOG in the presence or absence of TGF-β1 and changes in mRNA and protein were examined to infer the degree of transdifferentiation. ( A ) Western blot analysis of different groups COL1A1, HIF-1α, SMAD 3, and PSMAD3. β-actin was used as loading control. ( B ) Quantitative analysis of COL1A1, HIF-1α, P SMAD 3/ SMAD 3, and α-SMA is shown in A. ( C ) Different reagents were used to treat different groups of cells, and mRNA was extracted. mRNA expression was determined by qPCR and normalized to β-actin. * p < 0.05 vs. control group; # p < 0.05 vs. TGF-β1; % p < 0.05 vs. DMOG; & p < 0.05 vs. TGF-β1 + DMOG group Results are expressed as means ± SD. ANOVA was used to compare multiple groups and pairwise comparisons were compared by LSD-t test. A value of p < 0.05 was considered to be statistically significant.

Article Snippet: For IHC staining, refer to the papers [ ]; 5 μm thick sections were incubated with rabbit anti-mouse primary antibodies against HIF-1α (1:200, 20960-1-AP, ProteinTech) and COL1A1 (1:1000, GB11022-3, Servicebio), followed by horseradish peroxidase (HRP)–conjugated goat anti-rabbit IgG secondary antibody (1:8000, SA00001-2, Proteintech).

Techniques: Western Blot, Control, Expressing

Journal: International Journal of Environmental Research and Public Health

Article Title: Inhibition of HIF-1α Attenuates Silica-Induced Pulmonary Fibrosis

doi: 10.3390/ijerph19116775

Figure Lengend Snippet: A mouse PF model was established by nonexposure tracheal drip in C57BL/6N mice using saline or silica. The extent of PF in mice was inferred by Western blot, PCR, and IHC. Saline group (saline tracheal drip), silica group (silica particles tracheal drip), silica + NC group (silica particles tracheal drip, using saline intraperitoneal injection), and silica + KC7F2 group (silica particles, using KFC7F intraperitoneal injection). ( A ) Western blot analysis of COL1A1, HIF-1α,α-SMA, SMAD 3, and PSMAD3 in lung tissue of different groups. β-actin was used as loading control. ( B ) Quantitative analysis of COL1A1, HIF-1α, α-SMA P SMAD 3/ SMAD 3 is shown in A. ( C ) Quantitative analysis of the expression of mRNA. β-actin was used as an internal control. ( D ) H & E trichrome staining of mice lung tissue ( E ) Masson trichrome staining of mice lung tissue ( F ) IHC of COL1A1 protein. ( G ) IHC of HIF-1α protein. * p < 0.01 vs. saline group; # p < 0.01 vs. silica group. Results are expressed as means ± SD. ANOVA was used to compare multiple groups and pairwise comparisons were compared by LSD-t test. A value of p < 0.05 was considered to be statistically significant.

Article Snippet: For IHC staining, refer to the papers [ ]; 5 μm thick sections were incubated with rabbit anti-mouse primary antibodies against HIF-1α (1:200, 20960-1-AP, ProteinTech) and COL1A1 (1:1000, GB11022-3, Servicebio), followed by horseradish peroxidase (HRP)–conjugated goat anti-rabbit IgG secondary antibody (1:8000, SA00001-2, Proteintech).

Techniques: Saline, Western Blot, Injection, Control, Expressing, Staining